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. 2015 Feb 19;290(15):9896–9905. doi: 10.1074/jbc.M114.613422

FIGURE 1.

FIGURE 1.

Cytosolic protein carbonyls in phagocytic neutrophils. A, representative photograph taken from a cytospin preparation of human neutrophils co-incubated with S. aureus at a 20:1 ratio for 30 min. Phagocytosed S. aureus are observed within the cytosol of the neutrophil (arrow indicates one example). B, neutrophils were incubated alone (CTRL) or with S. aureus at ratios of 2:1, 10:1, 20:1, and 50:1 bacteria:neutrophil for 30 min, and the carbonyl content of the cytosol was measured by ELISA following nitrogen cavitation and centrifugation. C, cytosolic carbonyl levels in neutrophils co-incubated with S. aureus (20:1) for the various time intervals indicated. D, ELISA cytosolic carbonyl content of neutrophils co-incubated with S. aureus (20:1) (SA) or treated with 10 μm DPI (SA+DPI) or 1 mm azide (SA+AZ) prior to co-incubation with S. aureus for 30 min. The means and S.E. of three experiments are plotted. A significant difference (p < 0.05) from CTRL (B and C) or S. aureus (D) is indicated by *.