FIGURE 2.

Inhibition of protein carbonyl production in phagocytic neutrophils. A–C, neutrophils were incubated alone (CTRL) or treated with varying concentrations of butylated hydroxytoluene (BHT) (A), Trolox (B), or glutathione ethyl ester (GEE) (C) for 15 min prior to co-incubation with S. aureus at a 20:1 ratio (SA) for 30 min, and the carbonyl content of the cytosol was measured by ELISA following nitrogen cavitation and centrifugation. The means and S.E. of three experiments are plotted. A significant difference (p < 0.05) from the S. aureus neutrophils is indicated by *.