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. 2015 Feb 19;290(15):9896–9905. doi: 10.1074/jbc.M114.613422

FIGURE 3.

FIGURE 3.

HNE generates protein carbonyls in the cytosol of neutrophils. The isolated cytosol of DPI-treated phagocytic neutrophils (1 mg/ml) (CTRL) was pretreated with or without 10 mm NEM for 15 min before treatment with 2, 10, 50, or 200 μm HNE for 30 min. Protein carbonyl concentrations were measured by ELISA. The means and S.E. of three experiments are plotted. A significant difference (p < 0.05) between the control (CTRL) and HNE-treated cytosols without NEM is indicated by *. A significant difference (p < 0.05) between HNE-treated cytosols with and without NEM is indicated by #.