Table 2.
Effects of essential oils on populations of the ruminal bacteria in the phylum Firmicutes [only the OTUs with significant (P ≤ 0.05) changes in relative abundance are shown].
| Bacterial OTU | RDP ID | CTRL | ORO | GAO | PEO | SEM | P-value |
|---|---|---|---|---|---|---|---|
| Streptococcus_18 | S001093592 | 0.56a | 1.05a | 0.87a | 3.21b | 0.224 | <0.001 |
| Succiniclasticum_9 | S000566516 | 1.28b | 0.61a | 1.31b | 1.47b | 0.094 | 0.001 |
| Mitsuokella_17 | S000891012 | 0.71a | 0.68a | 0.56a | 3.39b | 0.278 | <0.001 |
| Papillibacter_10 | S000823615 | 0.48a | 0.51a | 0.40a | 2.59b | 0.299 | 0.002 |
| Papillibacter_13 | S001146016 | 1.05a | 1.33a | 0.97a | 2.15b | 0.115 | <0.001 |
| Ruminococcus_34 | S000991018 | 1.27a | 1.19a | 0.92a | 2.61b | 0.182 | 0.001 |
| Ruminococcus_59 | S001144527 | 0.67a | 0.73a | 0.63a | 1.55b | 0.100 | <0.001 |
| U_Ruminococcaceae_120 | S000990889 | 0.00a | 0.00a | 0.08a | 0.62b | 0.132 | 0.028 |
| U_Ruminococcaceae_132 | S000991199 | 2.92a | 6.80b | 6.47b | 8.21b | 1.119 | 0.050 |
| U_Ruminococcaceae_149 | S001159924 | 0.36ab | 0.49b | 0.24a | 1.37c | 0.050 | <0.001 |
| U_Ruminococcaceae_168 | S001144293 | 56.68a | 58.89a | 59.67a | 98.93b | 5.266 | 0.001 |
| U_Ruminococcaceae_20 | S000560533 | 0.75bc | 0.13a | 0.21ab | 0.83c | 0.179 | 0.050 |
| U_Ruminococcaceae_49 | S000616063 | 23.11c | 9.47ab | 21.48c | 3.77a | 3.733 | 0.025 |
| U_Ruminococcaceae_72 | S000650474 | 0.02a | 0.01a | 0.15a | 2.80b | 0.056 | <0.001 |
| Clostridium_2 | S000016649 | 0.38a | 0.27a | 0.62b | 1.43c | 0.070 | <0.001 |
| U_Peptococcaceae | S001382058 | 0.28a | 0.28a | 0.13a | 5.69b | 0.202 | <0.001 |
| Butyrivibrio_58 | S000438451 | 4.54ab | 3.04a | 5.48b | 6.97c | 0.754 | 0.034 |
| Syntrophococcus_1 | S000389024 | 79.98b | 46.12a | 67.50b | 49.46a | 4.734 | 0.003 |
| Roseburia_1 | S000561181 | 7.40b | 4.10a | 5.35ab | 16.22c | 0.822 | <0.001 |
| LIS_52 | S000926226 | 0.93a | 0.69a | 0.89a | 1.61b | 0.107 | 0.002 |
| LIS_61 | S001148837 | 0.21a | 0.10a | 0.08a | 0.89b | 0.053 | <0.001 |
| LIS_69 | S000823633 | 5.10b | 3.20a | 3.43a | 5.26b | 0.432 | 0.017 |
| LIS_80 | S000980403 | 19.15a | 12.46a | 21.68a | 40.59b | 4.227 | 0.008 |
| Pseudobutyrivibrio_8 | S000126942 | 1.38a | 0.57a | 1.16a | 5.76b | 0.616 | 0.001 |
| U_Lachnospiraceae_111 | S000806419 | 0.02a | 0.00a | 0.20a | 1.30b | 0.079 | <0.001 |
| U_Lachnospiraceae_137 | S000903858 | 2.45b | 0.94a | 2.06b | 0.67a | 0.245 | 0.002 |
| U_Lachnospiraceae_156 | S001144589 | 0.28a | 0.21a | 0.05a | 2.35b | 0.131 | <0.001 |
| U_Lachnospiraceae_198 | S001144193 | 0.06a | 0.00a | 0.11a | 7.42b | 0.120 | <0.001 |
| U_Lachnospiraceae_207 | S001144458 | 0.01a | 0.03a | 0.00a | 0.86b | 0.125 | 0.003 |
| U_Lachnospiraceae_38 | S000361672 | 0.95a | 0.57a | 0.82a | 2.24b | 0.255 | 0.007 |
| U_Lachnospiraceae_51 | S000566524 | 0.23a | 0.18a | 0.04a | 2.20b | 0.294 | 0.002 |
| U_Lachnospiraceae_77 | S000650410 | 16.21a | 15.33a | 20.82a | 53.82b | 6.130 | 0.006 |
| U_Lachnospiraceae_96 | S000821965 | 0.27a | 0.11a | 0.29a | 1.86b | 0.047 | <0.001 |
| U_Clostridiales_16 | S000361544 | 0.72a | 0.62a | 0.64a | 2.12b | 0.302 | 0.020 |
| U_Clostridiales_26 | S000566650 | 0.27a | 0.33a | 0.53a | 1.24b | 0.132 | 0.028 |
| U_Clostridiales_45 | S000650472 | 1.86a | 1.70a | 2.37a | 18.07b | 2.353 | 0.003 |
| U_Clostridiales_46 | S000653837 | 0.16a | 0.18a | 0.07a | 0.92b | 0.101 | 0.001 |
| U_Clostridiales_6 | S000335878 | 0.01a | 0.00a | 0.00a | 1.22b | 0.005 | <0.001 |
| U_Clostridiales_73 | S000888009 | 0.15a | 0.56b | 0.36ab | 0.78c | 0.069 | 0.001 |
| U_Clostridiales_85 | S000991126 | 1.80a | 0.91a | 1.49a | 17.18b | 0.290 | <0.001 |
| U_Clostridia_18 | S000566535 | 0.06a | 0.02a | 0.04a | 0.88b | 0.039 | <0.001 |
| Ad-C-8H | S002495906 | 3.80a | 5.07a | 3.49a | 16.21b | 2.471 | 0.019 |
U, unclassified; C, control (without any essential oil); GAO, garlic oil; PEO, peppermint oil; ORO, origanum oil; LIS, Lachnospiraceae incertae sedis.
Means followed by different letters in a row differ significantly (P ≤ 0.05) among the treatments.