Figure 2.
Current recordings of SCN10A variants. A) Representative whole-cell current traces of Nav1.8, using V1073 as reference construct, and mutant channels. Currents were recorded following a voltage step protocol with 5 mV increments from −70 mV to +50 mV, preceded with a −100 mV step. B) Current-voltage (I-V) relationship. The current is normalized to cell capacitance to provide a measure of Na+ current density. C) Peak current density at 15mV, Two-way ANOVA with Bonferroni post-tests was applied to test for significant differences. Mean ± SEM values are presented in Table 4. B, C) n=13–15 for each group. Asterisks indicate the voltages at which the parameters were statistically different versus V1073 *p<0.05, **p<0.01 and ***p<0.001.