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. Author manuscript; available in PMC: 2016 Apr 8.
Published in final edited form as: Neuron. 2015 Mar 19;86(1):264–275. doi: 10.1016/j.neuron.2015.02.036

Figure 5. Functionally Weakened PV-Circuits in Clock−/− Mice.

Figure 5

(A–C) Immature PV puncta onto pyramidal neurons in layer 4 of V1b in Clock−/− mice at CP (P29) (A). Cumulative plots for mean PV puncta size per soma (B) and PV puncta number per soma (C), and their mean values (insets) in Clock+/+ (90 cells from 4 mice) and Clock−/− mice (92 cells from 4 mice).

(D–F) Immature Syt2 puncta onto pyramidal neurons in layer 4 of V1b in Clock−/− mice at CP (P29) (D). Same analyses as in (B) and (C) in Clock+/+ (86 cells from 4 mice) and Clock−/− mice (102 cells from 4 mice). Scale bar, 5 μm. *P < 0.05; **P < 0.01; ***P < 0.001 (K–S test for cumulative plots; Mann-Whitney test for insets). Values are mean ± s.e.m.

(G–I) mIPSC traces from pyramidal neurons in layer 4 of V1b in Clock+/+ (21 cells from 3 mice) and Clock−/− mice (23 cells from 3 mice) at CP (P26–P28) (G). Cumulative plots for mIPSC amplitude (H) and inter-event interval (I) from all events, and their mean values from all cells (insets). ****P < 0.0001 (K–S test); *P < 0.05 (Mann-Whitney test for insets); ns, not significant. Values are mean ± s.e.m. See also Figures S4, S5, and Table S1.