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. Author manuscript; available in PMC: 2015 Oct 9.
Published in final edited form as: Nat Commun. 2015 Apr 9;6:6775. doi: 10.1038/ncomms7775

Figure 2. Borealin dimerization regulates CPC abundance at centromeres.

Figure 2

HeLa M cells were transfected with the indicated constructs and then exposed to thymidine for 24 hours to synchronize in S phase. At the time of thymidine removal, taxol was added in combination with AP20187 (+AP) or DMSO (−). Cells were fixed 16 hours later and analyzed by immunofluorescence. Intensity of centromere staining of Survivin (A), INCENP (B) and Aurora B (C) was quantified relative to the kinetochore marker CenpH (scale bar = 20μm). Bars represent standard deviation from averages of at least 50 centromere signals from 10 different cells per condition. *p<0.05 using a student’s t-test.