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. Author manuscript; available in PMC: 2015 Oct 9.
Published in final edited form as: Nat Commun. 2015 Apr 9;6:6775. doi: 10.1038/ncomms7775

Figure 6. Borealin dimerization is essential for localization near the kinetochore.

Figure 6

HeLa M cells were transiently transfected with Borealin-FKBP fusions, synchronized as shown and exposed to the homo-dimerizing agent AP20187 (+AP) or DMSO (−). All samples include 5Itu to reveal the kinetochore pool. Immunofluorescence was carried out using antibodies to HA to detect the Borealin-FKBP fusions, as well as Hec1 to stain the kinetochore. Examples of maximum projection images are show in the top three rows (scale bar = 10μm). Close-up images and line scans of individual sister kinetochores/centromeres from a single plane are also shown (scale bar = 0.5μm).