Figure 7. Monomeric but not dimeric Borealin_1-221-FKBP reduces CPC subunits at the kinetochore.

HeLa M cells were synchronized and treated as shown in the schematic to figure 5. However, when fixed, the cells were stained with antibodies to Survivin, INCENP, Aurora B, CenpH to mark the kinetochore, and HA to detect the Borealin-FKBP fusions. As in figure 5, all cells were exposed to 5Itu to remove the inner centromere pool and reveal the kinetochore pool. Examples of close-up confocal images of individual sister kinetochores/centromeres are shown for all conditions (scale bar = 0.5μm). Intensity of CPC family members was quantified and is shown relative to CenpH at individual kinetochores. At least 50 kinetochores from 10 different cells were analyzed. Bars represent standard deviation. *p<0.05 using a student’s t-test.