Figure 7.

Characterization of the flexibility of uPAR^WT and the mutated uPAR^H47C-N259C using EOM 2.0. (a) Size distributions (R _g) of uPAR^WT and uPAR^H47C-N259C, providing only a qualitative assessment through direct comparison of the distributions of the selected ensembles and the pool. (b) The metrics R _flex and R _σ enable characterization of the flexibility quantitatively, with R _flex = ∼82% and R _flex = ∼45%, for uPAR^WT and uPAR^H47C-N259C, respectively, reflecting a significant change in compactness of the particle upon mutation (with a threshold of randomness of ∼85% calculated from the pool).