Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Apr 15;142(8):1528–1541. doi: 10.1242/dev.119271

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Fig. 1. — Induction of lateral plate mesoderm (LM). (A) Schematic of HPSC differentiation to LM and epicardium. HPSCs were differentiated to early mesoderm using FGF2, Ly294002 and BMP4 for 36 h, and subsequently to LM with FGF2 and BMP4 for 3.5 days. (B) Analysis of LM/splanchnic mesoderm markers in LM by qRT-PCR. Student's t-test, ***P<0.001. (C) Percentage of ISL1⁺ and KDR⁺ cells in LM determined by flow cytometry. H9-derived neuroectoderm (NE) was used as a negative control. (D) The majority of LM cells immunostained positive for NKX2.5 and ISL1. Rabbit and mouse IgG isotypes were used as negative controls. Scale bars: 100 μm.