Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Apr 15;142(8):1528–1541. doi: 10.1242/dev.119271

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Fig. 5. — Epicardium-derived SMC differentiation in vitro. (A) Epithelial and mesenchymal marker expression in day 10 epicardial cells (EPI D10) and epicardium-derived SMCs (EPI-SMCs) after 3 (D3) and 6 (D6) days of differentiation with PDGF-BB and TGF-β1 (PT). ***P<0.001, **P<0.01. (B) SMC marker expression by qRT-PCR in EPI-SMCs differentiated with PT in the presence and absence of p160 Rho-kinase inhibitor (iROCK) after 3, 6 and 12 days of differentiation. Significant differences between PT and iROCK+PT are indicated in black. ***P<0.001, **P<0.01, *P<0.05. (C) EPI-SMCs after 12 days of PT treatment expressed mesenchymal (VIM) and SMC (ACTA2, CNN1 and TAGLN) markers, similar to human coronary artery SMCs (HCASMCs). SMC marker expression was absent in HUVECs. Scale bars: 100 μm. (D) Percentage of ACTA2⁺ and CNN1⁺ cells in H9 and BHX-derived EPI-SMCs. Mouse IgG isotypes served as negative controls.