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. 2015 Jan 9;36(4):420–428. doi: 10.1093/carcin/bgv001

Figure 3.

Figure 3.

Castration of male mice increases CD68- and CD8α-positive cells in FTC. (A) Immunostaining of CD68-positive macrophages. (B) Macrophage density comparison between castrated and sham-castrated males. Upper panel: macrophage densities in thyroid cancer samples. Lower panel: macrophage densities in liver samples. Mean macrophage densities ± SEM of seven random areas of representative liver samples. Error bars are ±SEM. *P < 0.05. (C) Representative immunofluorescence staining images of F4/80 (A), INOS (B), 4′,6-diamidino-2-phenylindole (C) and merged image (D). (D) Representative images of CD8α immunostaining. (E) Thyroid cancer CD8α-positive cell densities of castrated and sham-castrated males. Error bars are ±SEM. *P < 0.05. orchi = orchiectomy, oopho = oophorectomy.