Figure 2.

mPGES-1 deletion sensitizes the colonic mucosa to DSS-induced injury. (A) Experimental scheme for D14:WT (n = 7–10) and D14:KO (n = 3–16) mice treated with 0, 0.1, 0.5, 1 or 2% DSS and analyzed 2 days after DSS treatment. (B) Percent body weight change and spleen weights normalized to body weights were measured for each mouse. (C) Representative colon sections with ulcerated areas (demarcated by the brackets) in both genotypes with increasing concentrations of DSS. Sections were stained with H&E. Scale bars, 100 μm. (D) Quantification of affected areas within the entire length of colon is represented as a % ulceration. Bars indicate means ± SEM. Letters in the graph indicate significance measured by one-way analysis of variance with Bonferroni’s multiple comparison tests.