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. 2015 Mar 4;36(4):487–497. doi: 10.1093/carcin/bgv012

Figure 1.

Figure 1.

4OHT induces LoxP recombination and Rictor deletion in epidermis and primary keratinocytes of Cre-RictorL/L mice. (A) Schematic representation of the rictor allele targeted for K14-Cre-mediated recombination. (B) 7-week old Cre-RictorL/L mice were treated topically with 2-mg 4OHT or vehicle daily for 5 days. Top, PCR analysis of epidermal DNA harvested 7 days after final 4OHT treatment. ∆LoxP denotes primers specific to the recombined rictor allele. Bottom, immunoblot analysis of Raptor and Rictor in epidermal extracts harvested 7 days after final 4OHT treatment. Data are representative of two experiments. (C) Cre-RictorL/L and RictorL/L keratinocytes were harvested from 1–3 day-old pups and cultured in 4OHT (2nM) or vehicle for 3 days. Top, PCR analysis of primary keratinocyte DNA harvested 24h after final 4OHT treatment. ∆LoxP denotes primers specific to the recombined Rictor allele. Bottom, immunoblot analysis of Rictor in primary keratinocyte whole-cell lysates. Data are representative of 2–3 independent experiments.