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. 2015 Mar 6;36(4):459–468. doi: 10.1093/carcin/bgv017

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© The Author 2015. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 1. — IL-6 induces EMT changes and Fra-1 expresison in CRC cells. (A) Morphology of HT-29 cells treated with or without 50ng/ml IL-6 for 72h under phase contrast microscopy. Scale bar, 20 μm. (B) Immunofluorescent staining for E-cadherin and vimentin in HT-29 cells treated with IL-6 for 72h (nuclei stained with DAPI). Scale bar, 20 μm. (C) Western blots of E-cadherin and vimentin for HT-29 cells incubated with gradient concentrations of IL-6 for 72h. (D) Western blots of EMT markers with specific antibodies in SW480 cells exposed to IL-6 for 72h. (E) Relative mRNA levels of EMT regulators in HT-29 cells treated with IL-6 for 24h (normalized to GAPDH). *P < 0.05. (F) Western blots of Fra-1 and GAPDH from whole-cell lysates extracted from HT-29 and SW480 cells treated with 50ng/ml IL-6 for the indicated times.