Figure 1.

The effects of protease inhibitors (A), nucleoside reverse transcriptase inhibitors (B), non-nucleoside reverse transcriptase inhibitors (C) and the integrase inhibitor raltegravir (C) on the accumulation of tetraethylammonium in KCL22 cells. Results are given as tetraethylammonium concentrations in cells after 30 min incubation (μM, n = 9 replicates in SLC22A1-overexpressing KCL22 cells incubated with tetraethylammonium, n = 3 experimental replicates in mock transfected cells incubated with tetraethylammonium and n = 3 experimental replicates in SLC22A1-overexpressing KCL22 cells incubated with both tetraethyammonium and test compound, * p < 0.05, ** p < 0.01) ± SD. The IC₅₀ values of prazosin, efavirenz, and darunavir were determined in further experiments (D). Results are given as tetraethylammonium concentrations in cells after 30 min incubation (μM, n = 3 experimental replicates) ± SD.