Figure 1.

Representative photomicrographs of spinal cord longitudinal sections showing the rubrospinal tract region in the rostral side 24 hours after spinal cord injury.

The injured spinal cord immunostained for the axonal marker SMI31 (green) and for the autophagy proteins (red) ULK1 (A), Atg5 (B) or Atg7 (C). Arrows indicate examples of immunopositive axons. Scale bars: 20 μm. Insets: Higher magnification showing a retraction bulb stained for ULK1 (A), a retraction bulb with puncta-like structures positive for Atg5 (B; arrowheads) and an axon stained diffusely for Atg7 (C). Scale bars: 5 μm. Reproduced with permission from Brain Pathology, 2014, doi:10.1111/bpa.12170.