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. 2015 Feb 20;6(1):13. doi: 10.1186/s13287-015-0004-6

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© Lai et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Larger oocyte-like structures developed spontaneously from epithelial cells derived from human follicular fluid. (A) The volume of primary epithelial cells increased after 2 weeks of culturing. (B) Prominent nucleus and perinuclear accumulation of cytoplasmic organelles were present in large oocyte-like cells. Different sizes of oocyte-like structures after they were detached by trypsin after (C) 2 weeks , (D) 3 weeks , and (E) 4 weeks of culturing. (F) Blastocyst-like structures stained with 4′,6-diamidino-2-phenylindole developed after 6 weeks of culturing (arrowhead). Zona pellucida-like structures (arrow) were observed in epithelial cells post culture, indicating different stages of oocyte-like structure maturation. Scale bars: 200 μm (A), 100 μm (B, C), and 50 μm (D, E, F).