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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: J Drugs Dermatol. 2014 May;13(5):574–578.

Figure 3. Immunohistochemical characterization and quantification of HNE in UV-protected and UV-exposed human skin biopsy specimens.

Figure 3

A), C) UV-protected human skin sections stained with antibodies against HNE-adducts.

B), D) UV-exposed human skin sections stained with antibodies against HNE-adducts.

In UV-exposed skin samples there was a stronger staining for HNE in stratum corneum compared to the UV-protected skin (brown color, arrows). There is nuclear staining evenly observed in epidermis and dermis of both skin samples. Immunostaining is defined as low (A, C) and strong (B, D).

Original magnification A, B × 40 and C, D × 100.

E) Analysis of staining intensity scores for HNE. HNE staining of the stratum corneum was significantly increased in UV-exposed versus UV-protected skin (2.5 ± 0.22 versus 1.3 ± 0.12; p = <0.01). HNE staining was significantly increased in the epidermis of UV-exposed versus UV-protected skin (2.2 ± 0.18 versus 1.4 ± 0.17; p = <0.05). HNE staining was not significantly increased in the dermis of UV-exposed and UV-protected skin (1.9 ± 0.19 versus 1.2 ± 0.15). * p < 0.05, ***p < 0.01. Student t test. Data are shown as mean ± SEM; n=5.