Table 1. Characteristics of serogroup W isolates used in this study.

Molecular characterization was performed on these isolates by PCR amplification and sequencing of fHbp, porA, nadA and nhbA.

Isolate	Source	Origin	Year	fHbp variant	fHbp ID	PorA subtype	nadA allele	nhbA	Sequence type (ST)
1485*	carrier	Ghana	2003	2	23	P1.5,2	3	S	11
1487	carrier	Ghana	2003	2	23	P1.5,2	3	S	11
1489	carrier	Ghana	2003	2	23	P1.5,2	3	S	11
1491	carrier	Ghana	2003	2	23	P1.5,2	3	S	11
1494*	carrier	Ghana	2003	2	23	P1.5,2	3	S	11
1625*	case	Ghana	2003	2	23	P1.5,2	3	Y	11
1626	case	Ghana	2003	2	23	P1.5,2	3	S	11
1627*	case	Ghana	2003	2	23	P1.5,2	3	S	11
1628*	case	Ghana	2003	2	23	P1.5,2	3	S	11
1629	carrier	Ghana	2004	2	23	P1.5,2	3	N	11
1630*	carrier	Ghana	2004	2	23	P1.5,2	3	S	11
1632	carrier	Ghana	2004	2	23	P1.5,2	3	Y	11
1634*	carrier	Ghana	2004	2	23	P1.5,2	3	Y	11
1636	carrier	Ghana	2004	2	23	P1.5,2	3	Y	11
1681*	case	Ghana	2003	2	23	P1.5,2	3	Y	11
1682*	case	Ghana	2003	2	23	P1.5,2	3	S	11
1683*	case	Ghana	2003	2	23	P1.5,2	3	S	11
1846*	carrier	Ghana	2004	2	23	P1.5,2	3	Y	11
1848	carrier	Ghana	2004	2	23	P1.5,2	3	Y	11
1857*	carrier	Ghana	2004	2	23	P1.5,2	3	Y	11
1888	carrier	Ghana	2004	2	23	P1.5,2	3	S	11
1903*	case	Ghana	2004	2	23	P1.5,2	3	Y	11
1973	carrier	Ghana	2004	2	23	P1.5,2	3	Y	11
2039*	case	Burkina Faso	2008	2	22	P1.5,2	3	Y	11
2252*	case	Burkina Faso	2008	2	22	P1.5,2	NA	S	11
2716	carrier	Burkina Faso	2004	2	22	P1.5,2	3	Y	11
2719*	carrier	Burkina Faso	2004	2	22	P1.5,2	3	Y	11
2841	carrier	Burkina Faso	2005	2	22	P1.5,2	NA	Y	11
2855	carrier	Burkina Faso	2005	2	22	P1.5,2	NA	S	11
2882	carrier	Burkina Faso	2009	2	22	P1.5,2	NA	S	11
2959*	carrier	Burkina Faso	2009	2	22	P1.5,2	NA	Y	11

*Isolate used for fHbp protein expression analysis.

NA: Inactive nadA due to insertion sequence IS1301.

S: nhba gene with stop codon.

Y: Full length nhba gene

N: No gene product obtained by PCR