Table 2. Primers used for PCR amplification and sequencing of the genes fHbp, porA, nadA snd nhbA.
| Target
gene |
Primer
designation |
5′-3′ nucleotide sequence | Reference |
|---|---|---|---|
| fHbp | A1 (Fw)
B2 (Rv) |
GACCTGCCTCATTGAT
CGGTAAATTATCGTGTTCGTACGGC |
[
17]
[ 17] |
| porA | 210 (Fw)
H (Rv) EI (Fw) 103L (Rv) |
ATGCGAAAAAAACTTACCGCCCTC
CGCATATTTAAAGGCATAG CCAGCCAGGCCATTGATCC AACGGATACGTCTTGCTC |
[
27]
This study This study [ 27] |
| nadA | NadAF (Fw)
NadAR (Rv) |
AACACTTTCCATCCAAAG
TTACCACTCGTAATTGACG |
[
23]
[ 23] |
| nhbA | Fw
Rv |
GGCGTTCAGACGGCATATTTTTACA
GGTTTATCAACTGATGCGGACTTGA |
[
20]
[ 20] |
Fw: forward; Rv: reverse.