Table 3. Conditions used for PCR amplification of the genes fHbp, porA, nadA and nhbA.

	fHbp	porA	nadA	nhbA
PCR profile	94ºC, 4 minutes 35 cycles: 94ºC, 40 seconds 58ºC, 40 seconds 68ºC, 40 seconds Final extension: 72°C, 5 minutes	94ºC, 5 minutes 30 cycles: 94ºC, 1 minute 55ºC, 1 minute 72ºC, 30 seconds Final extension: 72°C, 5 minutes	94ºC, 5 minutes 30 cycles: 94ºC, 1 minute 55ºC, 1 minute 72ºC, 1 minute 30 seconds Final extension: 72°C, 5 minutes	94ºC, 4 minutes 35 cycles: 94ºC, 1 minute 55ºC, 1 minute 72ºC, 1 minute Final extension: 72°C, 5 minutes
Reference	[ 17]	[ 27]	[ 23]	[ 20]