Table 1. Primer sequences for each SNP and PCR conditions.
Gene | Gene location | SNPs | Primer pairs | Product length | Anneling temperature (°C) | Extendding time(s) | |
---|---|---|---|---|---|---|---|
Dectin-1 |
Intron |
rs17206002 rs3901533 rs11053613 rs3901532 |
F |
TGACTACAATAATCAGGACACTACC |
777 bp |
64 |
47 |
R |
TAGCCATTGTCTTCTCCTCCA |
||||||
DC-SIGN |
Promoter |
rs4804803 rs2287886 |
F |
ATTATGATTCTGCCCCAACTC |
424 bp |
63 |
27 |
R |
CAGCTTTTATTTCCCACCCT |
||||||
DC-SIGN | Promoter | rs735239 rs735240 | F |
TAGATGGTGGGGCTGAGACT |
407 bp | 62 | 25 |
R | GCATACAGAAACCCCGTTGT |