Figure 8. The Nematostella HoxE and mouse HoxB8 proteins interact with several Drosophila TFs.
(A) Schematic representations of the Hox proteins and the corresponding animal phylogeny. The percentage of sequence identity is given in comparison to AbdA. (B) Interactome between mouse HoxB8 and the 35 Drosophila TFs. (C) Interactome between Nematostella HoxE and the 35 Drosophila TFs. Colour code and representation are as in Figure 3. See also Figure 8—figure supplements 1–5.
Figure 8—figure supplement 1. BiFC between Drosophila TFs and the mouse HoxB8 protein.
Illustrative pictures are provided for each TF as indicated. No picture was taken in absence of BiFC.
Figure 8—figure supplement 2. BiFC between Drosophila TFs and the Nematostella HoxE protein.
Illustrative pictures are provided for each TF as indicated. No picture was taken in absence of BiFC.
Figure 8—figure supplement 3. BiFC between Drosophila TFs and HX-mutated HoxB8.
Illustrative pictures are provided for each TF as indicated. BiFC was performed with TFs that were negative with wild type HoxB8. The HX mutation leads to five new interactions among the eight tested TFs (as seen for Apontic, Bagpipe, Biniou, Empty spiracles and Suppressor-of-Hairless). No picture was taken in absence of BiFC.
Figure 8—figure supplement 4. BiFC between Drosophila TFs and HX-mutated HoxE.
Illustrative pictures are provided for each TF as indicated. BiFC was performed with TFs that were negative with wild type HoxE. The HX mutation leads to seven new interactions among the twelve tested TFs (as seen for Bagpipe, Empty spiracles, Eyes absent, Knot, Pangolin, Seven up and Suppressor-of-Hairless). No picture was taken in absence of BiFC.
Figure 8—figure supplement 5. Drosophila AbdA, mouse HoxB8 and Nematostella HoxE interact with several common TFs in vivo.
Colour code and interaction representation are as in Figure 3.