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. Author manuscript; available in PMC: 2016 Feb 26.
Published in final edited form as: Cell. 2015 Feb 26;160(5):842–855. doi: 10.1016/j.cell.2015.02.004

Figure 1. CRTC-1 uncouples AMPK-mediated longevity from other pleiotropic phenotypes.

Figure 1

(A) AMPK activation (CA-AAK-2) extends lifespan in a wild type C. elegans background. See Table S1 for lifespan statistics.

(B) CA-AAK-2 suppresses growth. Image of 5 wild type (left) and 5 CA-AAK-2 (right) adult worms.

(C) Eggs laid per worm over successive 12 h periods. Data are mean ± SD for n=25-30 animals; * denotes p<10−4 via t-test.

(D) CA-AAK-2 fails to promote longevity in a CRTC-1S76A,S179A mutant background.

(E) CRTC-1S76A,S179A does not suppress AMPK-mediated effects on growth.

(F) Eggs laid per worm over successive 12 h periods (data are mean ± SD for n=19–30 animals; * denotes p<10−4 via t-test.

(G and H) Survival curves of AMPK and CRTC-1S76A,S179A transgenic animals exposed to heat stress at 33°C. N=60-100 worms. p<10−5 via Log-rank (Mantel-Cox) analysis.

(I) CRTC-1 is a longevity-specific AMPK target that uncouples growth, reproduction, and stress resistance from lifespan extension.