Table 2.
Effect of nitrogen sources on protease production by B. circulans MTCC 7942.
Nitrogen supplementation (1%)# | Protease activity (U/mL ± SD) |
Specific activity (U/mg ± SD) |
---|---|---|
Feather meal | 69.5 ± 6.9 | 222.6 ± 21 |
Bovine serum albumin | 216.5 ± 18.4 | 635.9 ± 55 |
Beef extract | 300.6 ± 12.0 | 905.7 ± 41 |
Casein | 320.7 ± 6.0 | 884.2 ± 114 |
Casamino acids | 329.1 ± 13.3 | 966 ± 31 |
Yeast extract | 330.1 ± 23.8 | 875.8 ± 163 |
Gelatin | 339.2 ± 10.0 | 1063.0 ± 39 |
Peptone | 357.0 ± 10.5 | 1007.0 ± 75 |
Soybean meal | 397.0 ± 7.6 | 1164.0 ± 33 |
#Basal medium containing (g/L): starch, 20; KH2PO4, 1; MgSO4·7H2O, 0.05; CaCl2, 1; Na2CO3, 8 and cultural parameters (pH 10.0) at 37°C and 100 rpm.