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. Author manuscript; available in PMC: 2016 Feb 5.
Published in final edited form as: Mol Cell. 2015 Feb 5;57(3):537–551. doi: 10.1016/j.molcel.2015.01.002

Figure 3. Activation of Ras or MAPK signaling leads to Mek-dependent mitochondrial fragmentation.

Figure 3

(A) Mitochondrial morphologies of HEK-TtH cells stably expressing mito-YFP and HRasG12V and treated with either DMSO or 200nM PD325901 for 24 hours. Green: mito-YFP; Blue: DAPI. Scale Bar = 20μm. (B) Quantitation of mitochondrial morphologies observed in cells described in (A). n>50 cells, blindly scored by 5 people, 3 independent experiments; Error bar: S.E.M of mean percentages from 1 representative experiment. (C–F) Mitochondrial morphologies of HEK-TtH cells transfected with mito-YFP plus either vector, Raf-22W or Mek-DD and treated with either DMSO or 200nM PD325901 for 24 hours as indicated. Green: mito-YFP; Blue: DAPI. Scale Bar = 20μm; Quantitation of mitochondrial morphologies: n>50 cells, blindly scored by 5 people, 3 independent experiments; Error bar: S.E.M of mean percentages from 1 representative experiment.