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. Author manuscript; available in PMC: 2015 Apr 10.
Published in final edited form as: J Med Genet. 2014 Nov 20;52(2):104–110. doi: 10.1136/jmedgenet-2014-102677

Figure 3.

Figure 3

SPECC1L-T397P and SPECC1L-G1083S mutant proteins are defective in stabilising microtubules. U2OS cells were transfected with wildtype or mutant green fluorescent protein (GFP)-tagged Specc1l expression constructs. Wildtype SPECC1L-GFP expression (A; green) stabilises a subset of microtubules that colocalise with acetylated-α-tubulin (B, C; red). Compared with wildtype protein (A–C), both SPECC1L-T397P (D–F) and SPECC1L-G1083S (G–I) mutant proteins fail to stabilise microtubules efficiently and show a punctuate expression pattern, similar to SPECC1L-Q415P mutant protein ( J–L). SPECC1L-ΔCHD shows a diffuse expression pattern with near complete absence of microtubule stabilisation (M–O). Nuclei are stained with DAPI (blue).