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. 2015 Apr 10;10(4):e0123419. doi: 10.1371/journal.pone.0123419

Fig 4. Western blot of oleosin prototypes isolated from roasted peanuts and purified by preparative electrophoresis.

Fig 4

Fractions obtained by preparative electrophoresis containing the prototypes 1/2 (A) and prototypes 3/4 (B) were pooled, subjected to SDS-PAGE and subsequent immunoblotting. M, molecular mass marker; G, Gold staining; CS, Coomassie staining; A, polyclonal anti-oleosin antibody; P1–P4, allergic patients’ sera; P°, allergic individual (not to peanut) with respiratory symptoms; P*, healthy control; C1, secondary antibody control (mouse anti-human IgE antibody); C2, secondary antibody control (goat anti-rabbit IgG antibody).