Figure 1.

Fresh weight (g) variation of Echium acanthocarpum transformed hairy roots cell line E1.5 cultured in three different growth media and temperatures. Values represent the mean of three replicates (n = 3)  ±  SD. Depending on the culture temperature 25°C (culture B1) or 15°C (cultures C1–C4), the sampling points varied as T1 = 5 days for culture B1 and 15 days for cultures C1–C4; T2 = 10 days for culture B1 and 25 days for cultures C1–C4; T3 = 15 days for culture B1 and 35 days for cultures C1–C4; T4 = 20 days for culture B1 and 45 days for cultures C1–C4; T5 = 25 days for culture B1 and 55 days for cultures C1–C4; and T6 = 30 days for culture B1 and 65 days for cultures C1–C4.