(b) Data of sampling point 5

Sampling point 5	Culture B1	Culture C1	Culture C2	Culture C3	Culture C4
TL (mg/g DW)		35.58 ± 7.33	28.59 ± 6.17	42.96 ± 5.50	28.34 ± 4.17
PC	—	7.19 ± 1.06	5.72 ± 1.64	4.96 ± 1.24	8.54 ± 0.97
PS + PI	—	2.80 ± 2.09	6.27 ± 2.35	3.18 ± 1.80	5.09 ± 1.45
PG	—	5.12 ± 0.70	9.86 ± 1.79	9.96 ± 2.23	12.91 ± 1.96
PE	—	8.79 ± 1.59	9.32 ± 1.47	8.96 ± 2.87	13.97 ± 1.95
Polar lipid	—	23.91 ± 5.42	31.17 ± 6.97	27.06 ± 4.92	40.51 ± 6.09
Neutral lipid	—	71.97 ± 7.95	64.39 ± 6.89	72.94 ± 4.92	59.49 ± 6.09
unknown		4.11 ± 2.53	4.43 ± 0.21	1.41 ± 2.45	12.30 ± 1.69

^*Significant differences (P ≤ 0.05) between cultures given by Student's t-test when comparing the mean of percentages of the lipid classes for cultures B1 and C1.

^a,bSignificant differences of TL values, also showing homogeneous subclusters as indicated by Tuckey's test when comparing the mean of TL values.

TL: total lipid; PC: phosphatidylcholine; PS + PI: sum of phosphatidylserine and phosphatidylinositol; PG: phosphatidylglycerol; PE: phosphatidylethanolamine.

Percentage values were transformed by the arcsin transformation prior to statistical analysis.

Values represent the mean of three independent experiments (n = 3) ± SD.

Entry of culture B1 for sampling point 5 is empty because data were collected only for sampling 4.