Table 2.
Detection of microbial diversity in ATAD sludge at different stages of ATAD treatment. PCR amplification using Archaea-, Eukarya-, Bacteria-, and Fungal-specific primers as listed in Table 1 was used.
| Origin of the extracted DNA | PCR amplification with genera- and domain-specific primers | |||
|---|---|---|---|---|
| Eukarya | Archaea | Bacteria | Fungi | |
| Inlet type I (sewage) | + | + | + | + |
| Inlet type II (secondary sludge) | + | − | + | + |
| Thickened inlet sludge | + | + | + | + |
| Reactor 1A ATAD (2 hours of operation) | + | − | + | + |
| Reactor 1A ATAD (4 hours of operation) | − | − | + | + |
| Reactor 1A ATAD (16 hours of operation) | − | − | + | − |
| Rector 2A ATAD (4 hours of operation) | − | − | + | − |
| Reactor 2A ATAD (23 hours of operation) | − | − | + | − |
| Biosolids (9 days and storage) | + | − | + | + |
Positive detection is indicated as (+) ve whereas lack of amplification is indicated as (−) ve.