Table 1.

CCD showing experimental and regression model predicted CMCase activity (U/mL) and cell growth (g/L).

Run no.	pH	Temp (°C)	Agitation (rpm)	CMCase activity (U/mL)		Cell growth (g/L)
				Measured	Predicted	Measured	Predicted
1	0 (7.0)	0 (37)	0 (180)	0.429 ± 0.05	0.437	3.16 ± 0.01	3.18
2	1 (7.6)	−1 (33)	1 (215)	0.311 ± 0.01	0.335	2.30 ± 0.05	2.39
3	−1 (6.4)	1 (41)	−1 (144)	0.499 ± 0.02	0.512	1.87 ± 0.12	1.85
4	α (8.0)	0 (37)	0 (180)	0.560 ± 0.01	0.503	2.74 ± 0.09	2.53
5	0 (7.0)	0 (37)	−α (120)	0.500 ± 0.08	0.490	2.08 ± 0.01	2.14
6	−α (5.9)	0 (37)	0 (180)	0.355 ± 0.05	0.360	1.98 ± 0.04	2.10
7	0 (7.0)	0 (37)	0 (180)	0.442 ± 0.02	0.437	3.23 ± 0.14	3.18
8	0 (7.0)	0 (37)	0 (180)	0.436 ± 0.03	0.437	3.19 ± 0.06	3.18
9	0 (7.0)	0 (37)	0 (180)	0.422 ± 0.06	0.437	3.16 ± 0.02	3.18
10	1 (7.6)	1 (41)	−1 (144)	0.532 ± 0.02	0.538	2.08 ± 0.08	2.06
11	1 (7.6)	1 (41)	1 (215)	0.322 ± 0.02	0.374	2.73 ± 0.05	2.93
12	0 (7.0)	0 (37)	0 (180)	0.448 ± 0.01	0.437	3.14 ± 0.04	3.18
13	−1 (6.4)	−1 (33)	−1 (144)	0.217 ± 0.07	0.202	1.88 ± 0.02	1.75
14	0 (7.0)	0 (37)	0 (180)	0.435 ± 0.04	0.437	3.19 ± 0.21	3.18
15	1 (7.6)	−1 (33)	−1 (144)	0.281 ± 0.11	0.325	1.87 ± 0.05	2.01
16	−1 (6.4)	1 (41)	1 (215)	0.334 ± 0.01	0.327	2.73 ± 0.02	2.67
17	0 (7.0)	0 (37)	α (240)	0.386 ± 0.05	0.344	3.30 ± 0.04	3.15
18	0 (7.0)	α (44)	0 (180)	0.385 ± 0.01	0.365	2.15 ± 0.18	2.13
19	0 (7.0)	−α (30)	0 (180)	0.103 ± 0.14	0.071	1.65 ± 0.05	1.58
20	−1 (6.4)	−1 (33)	1 (215)	0.161 ± 0.03	0.192	2.00 ± 0.01	2.08

Values are mean ± SE (n = 3).