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. 2015 Apr 10;10(4):e0122936. doi: 10.1371/journal.pone.0122936

Fig 4. The processing of AtbZIP60s is a dynamic process.

Fig 4

(A) Wild-type Arabidopsis seedlings (7-days-old) were treated with DTT (2 mM) for two hours then media was changed to fresh liquid 0.5x MS plus 1% sucrose for two additional hours and finally treated with DTT (2 mM) for another two hours. The complete experiment was extended for six hours. Total RNA samples were obtained at the indicated time points. RT-PCR products generated from total RNA were analyzed by CE-LIF. Identical procedures were performed for treatments with tunicamycin (Tm; 5 μg/mL) (B) or salicylic acid (SA; 0.5 mM) (C). In the case of heat treatment, instead of changing the culture media, flasks with seedlings exposed to high temperature (42°C) were transfered to room temperature (RT) for two hours and re-exposed to high temperature (42°C) during two more hours (D). All calculations were performed as described in materials and methods section. Data are representative of three independent experiments. Data are shown as mean ± SD.