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. 2015 Apr 10;11(4):e1005159. doi: 10.1371/journal.pgen.1005159

Fig 6. Rra1 is a membrane protein and is conserved through Basidiomycete fungi.

Fig 6

(A) C. neoformans Rra1 and its predicted basidiomycete orthologs contain 7–8 predicted transmembrane domains (closed boxes). C. neoformans CNAG_03488 (Rra1), C. gattii CGB_G5320C, Tremella mesenterica TREME_69388, Ustilago maydis um00299, Puccinia graminis PGTG_03106 predicted protein sequences are modeled. The TM domains were predicted using The TMPred TM prediction server [38] The % identity to the transmembrane region of C. neoformans Rra1 was determined by comparing 1–290 of the predicted Rra1 amino acid sequence to each of the predicted orthologs. (B) C. gattii rra1Δ and rim101Δ mutants are sensitive to pH 8. (C) Cg rra1Δ and rim101Δ have a capsule defect. Capsules visualized with India Ink after 24 hr incubation in tissue culture media. Scale bar = 5 μm.