. 2015 Apr 10;10(4):e0121043. doi: 10.1371/journal.pone.0121043

Table 1. Influence of tRNA on GluRS/Glu-AMS binding at 30°C.

	E. coli GluRS	E. coli GluRS + E. coli tRNA^Glu	E. coli GluRS + S. cerevisiae tRNA^Phe
n^a	0.9998 ± 0.0094	1.0018 ± 0.0041	0.9902 ± 0.0410
ΔH_b (cal/mol)	-5041 ± 63	-7990 ± 62	-5173 ± 279
ΔS_b (cal/mol·K)	13.19 ± 0.16	10.52 ± 0.08	7.92 ± 0.46
ΔG_b (cal/mol)	-9056 ± 38	-11304 ± 209	-8544 ± 309
-TΔS_b (cal/mol)	-3996 ± 50	-3186 ± 25	-2398 ± 140

n = stoichiometry coefficient (number of moles of Glu-AMS bound per mole of GluRS monomer), ΔH_b = reaction enthalpy, ΔS_b = reaction entropy, ΔG_b = reaction energy (calculated with the formula ΔG_b = -RT Ln K _b, where R = 1.987 cal/mol·K).

All values and errors in this table were obtained by weighting by inverse variance [32], except for ΔG_b values and errors, obtained by simple average and standard error calculations.

Raw data and calculated values for each separated ITC runs are shown in S1 Table.