Figure 1. Spatially distinct pools of m⁶A in mRNAs.

Metagene analysis of MeRIP-Seq data shows that m⁶A is enriched in discrete regions of mRNA transcripts³. In this approach, each m⁶A peak in the transcriptome was mapped to a virtual transcript based on its position in the mRNA in which it is found. The metagene profile represents an overall frequency distribution of m⁶A residues along the entire mature transcript body. Shown is an idealized metagene profile based on published MeRIP-Seq datasets³. m⁶A residues can be found at the first encoded residue ,often as a dimethylated nucleotide (m⁶Am). m⁶A is also found at each of the other indicated positions in mRNAs, with a particularly prominent enrichment near the stop codon in mRNAs.