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. 2015 Apr 10;10(4):e0123018. doi: 10.1371/journal.pone.0123018

Fig 2. Effect of FLNA on PC2 degradation.

Fig 2

A, left panel, representative data from WB showing PC2degradation in M2 and A7 cells using cycloheximide (CHX) to block protein synthesis for various time (in hr). Right panel, data on PC2 degradation from 4 independent experiments were normalized, averaged and fitted with exponential decay curves Y(t) = 2-t/τ, where τ represents the protein half-life. B, representative data showing degradation of GFP-PC2 (upper panel), GFP-PC2C (middle panel) and GFP-PC2N (lower panel) transiently expressed in A7 cells with FLNA KD or control (Ctrl). C, representative data showing proteasome-dependent inhibition of degradation of GFP-PC2 stably expressed in A7 and M2 cells by MG-132. Cells were treated with MG-132(10 μM) or DMSO (negative control) for 4 hr before proceeding to WB with FLNA and PKD2 antibodies.