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. 2015 Apr 10;10(4):e0123018. doi: 10.1371/journal.pone.0123018

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© 2015 Wang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 3 — A, left panel, cell surface biotinylation was performed to assess GFP-PC2 PM expression in A7 and M2 cells. Shown are data from biotinylated (BT) and flow-through (FT) lysates. Right panel, comparison between averaged GFP-PC2 surface membrane expression normalized by Na/K ATPase in M2 and A7 cells (N = 3; p = 0.01). B, biotinylation assays using native A7 (Ctrl) and A7 PC2 stable cells to determine the effect of surface PC2 on the recruitment of FLNA to the PM. C, biotinylation assays using M2 cells with and without transient expression of wild type FLNA transient expression to determine the rescue effect of FLNA on M2 cells.