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. 2015 Apr 10;10(4):e0123018. doi: 10.1371/journal.pone.0123018

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© 2015 Wang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — All data are from three or more independent experiments. A, representative data from co-IP showing interaction of GFP-PC2 with FLNA and FLNAC in M2 and A7 cells. B, representative data showing the effect of blocking peptide FLNAC on the FLNA-PC2 interaction in A7 cells. C, Far WB showing the competition of FLNAC with PC2C for binding to FLNA. Lysates of M2 and A7 cells (stably expressing GFP-PC2) were separated by SDS-PAGE and transferred to nitrocellulose membrane. Proteins were denatured, renatured and then incubated with purified GST-PC2C and none (left panel) or His-FLNAC (right panel). Bound protein was detected by PC2 (H-280) antibody. The arrow (←) indicates PC2C signal detected at the site of FLNA. The star (*) indicates stably expressed GFP-PC2 signal, as a control.