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. 2015 Apr 10;10(4):e0123018. doi: 10.1371/journal.pone.0123018

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© 2015 Wang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 5 — A, representative biotinylation data showing the effect of FLNAC on the PM (biotinylation) and intracellular (FT) GFP-PC2 expression in M2 and A7 cells. B, averaged PC2 PM expression in A7 cells showing the effect of FLNAC over-expression. FLNAC reduced PC2 PM expression to 46% ± 12% (N = 3; p < 0.001) of the control level. C, representative data showing the effect of FLNAC on the total PC2 expression in GFP-PC2 stably expressing A7 (left panel) (N = 3; p = 0.01) and M2 cells (right panel) (N = 3; p = 0.16). HSP60 was used as a loading control. D, WB data showing the effect of FLNAC on the endogenous PC2 level in HeLa (left panel), HEK293 (middle panel) and IMCD (right panel) cells detect by antibody H-280. β-actin was used as a loading control (N = 3).