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. 2015 Apr 10;10(4):e0123018. doi: 10.1371/journal.pone.0123018

Fig 7. Ca dependence of the physical interaction between PC2 and FLNA.

Fig 7

A, co-IP experiments showing Ca-dependent binding between FLNA and PC2 in HeLa cells. HeLa cell lysate with GFP-PC2 transient over-expression was equally split into three samples and added with none (Ctrl), 1 mM Ca or 1 mM EGTA (all final concentrations). FLNA (E-3) and GFP (EU4) antibodies were used for IP and IB detection, respectively. B and C, co-IP experiments showing the effect of Ca on the FLNA-PC2 binding in GFP-PC2 stably expressing IMCD (B) and HEK293 (C) cells with and without FLNA KD.