Table 1. Striatal concentrations of monoamines, 1 hour after injection of 5 mg/kg L-DOPA with or without 1.25 mg/kg carbidopa in hemi-parkinsonian rat after unilateral transduction with AAV2-GFP or AAV2-hAADC vector.
| Group | Treatment | Monoamines (ng/mg protein) | |||||
|---|---|---|---|---|---|---|---|
| L-DOPA | DA | DOPAC | HVA | 5-HT | 5-HIAA | ||
| Lesioned striatum | |||||||
| AAV2-GFP | LD/carbi + | 1.15 ± 0.30 | 1.89 ± 0.07 | 0.97 ± 0.16 | 1.71 ± 0.06 | 6.22 ± 0.80 | 6.82 ± 0.5 |
| LD/carbi - | 1.22 ± 1.30 | 0.97 ± 0.12 b | 0.27 ± 0.03 b | 0.67 ± 0.18 b | 7.92 ± 0.60 | 6.51 ± 0.6 | |
| AAV2-hAADC | LD/carbi + | 0.32 ± 0.12 a | 1.87 ± 0.12 | 1.39 ± 0.15 a | 2.25 ± 0.05 a | 5.96 ± 0.53 | 6.52 ± 0.8 |
| LD/carbi - | 0.17 ± 0.15 | 1.11 ± 0.12 b | 0.36 ± 0.04 b | 0.65 ± 0.14 b | 7.89 ± 1.00 | 6.03 ± 0.4 | |
| Un-lesioned striatum | |||||||
| AAV2-GFP | LD/carbi + | 0.45 ± 0.05 | 153.9 ± 5.70 | 15.5 ± 0.60 | 11.3 ± 0.40 | 3.12 ± 0.30 | 3.68 ± 0.2 |
| LD/carbi - | 0.57 ± 0.17 | 111.5 ± 11.5 b | 12.4 ± 2.80 | 7.1 ± 1.10 b | 3.43 ± 0.40 | 3.51 ± 0.5 | |
| AAV2-hAADC | LD/carbi + | 0.61 ± 0.08 | 148.8 ± 11.1 | 12.7 ± 1.32 | 9.6 ± 0.71 | 3.86 ± 0.51 | 4.15 ± 0.4 |
| LD/carbi - | n.d. | 132.3 ± 10.6 | 10.9 ± 1.56 | 7.3 ± 0.89 b | 3.10 ± 0.32 | 3.49 ± 0.3 | |
Data are expressed as mean and S.E.M. (n = 5 per group)
Confidence intervals are indicated as follows:
a p<0.05 compared to AAV2-GFP treated with L-DOPA/carbidopa + (LD/carbi +) group
b p<0.01 compared to LD/carbi + groups