Table 2. Metabolic rate of striatal monoamines, 1 hour after injection of 5 mg/kg L-DOPA with or without 1.25 mg/kg carbidopa in hemi-parkinsonian rat after unilateral transduction with AAV2-GFP or AAV2-hAADC vector.
| Group | Treatment | Metabolic rate of monoamines | |||
|---|---|---|---|---|---|
| DOPAC / DA | HVA / DA | DOPAC+HVA / DA | 5-HIAA / 5-HT | ||
| Lesioned striatum | |||||
| AAV2-GFP | LD/carbi + | 0.52 ± 0.08 | 0.81 ± 0.04 | 1.33 ± 0.10 | 1.05 ± 0.10 |
| LD/carbi - | 0.27 ± 0.01 b | 0.65 ± 0.13 b | 0.92 ± 0.13 b | 0.82 ± 0.05 b | |
| AAV2-hAADC | LD/carbi + | 0.74 ± 0.02 a | 1.27 ± 0.09 a | 1.95 ± 0.10 a | 1.12 ± 0.05 |
| LD/carbi - | 0.32 ± 0.06 b | 0.61 ± 0.12 b | 0.92 ± 0.15 b | 0.94 ± 0.09 b | |
| Un-lesioned striatum | |||||
| AAV2-GFP | LD/carbi + | 0.10 ± 0.01 | 0.07 ± 0.01 | 0.17 ± 0.01 | 0.98 ± 0.56 |
| LD/carbi - | 0.11 ± 0.01 | 0.07 ± 0.02 | 0.17 ± 0.03 | 0.94 ± 1.15 | |
| AAV2-hAADC | LD/carbi + | 0.08 ± 0.01 | 0.06 ± 0.01 | 0.14 ± 0.02 | 1.07 ± 0.06 |
| LD/carbi - | 0.08 ± 0.01 | 0.05 ± 0.01 | 0.13 ± 0.01 | 1.06 ± 0.08 | |
Data are expressed as mean and S.E.M. (n = 5 per group)
Confidence intervals are indicated as follows:
a p<0.05 compared to AAV2-GFP treated with L-DOPA/carbidopa + (LD/carbi +) group
b p<0.01 compared to LD/carbi + groups