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. 2015 Apr 10;10(4):e0122953. doi: 10.1371/journal.pone.0122953

Fig 3. Replication of 3′PPT and cPPT mutant viruses.

Fig 3

(A) Introduction of single and double mutations into the 3′PPT or cPPT region in an NL4-3 backbone through yeast based/HIV-1 cloning technology [33]; (B) Replication of various PPT mutants, as well as Δnef mutant (with premature stop codon in nef but outside of PPT region) in U87.CD4.CXCR4 cells. The input viruses were normalized according to RT activity.