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. 2015 Apr 10;10(4):e0122953. doi: 10.1371/journal.pone.0122953

Fig 6. In vitro PPT RNA/DNA hybrids cleavage assay.

Fig 6

(A) PPT RNA-DNA hybrid substrates (wild type, G(-1)A, T(-16)A, and G(-1)A/T(-16)A double mutants) were subjected to RNase H-mediated cleavage by HIV-1 RT in the absence of nucleotides. Reactions were terminated at 0, 1, 3, 10, or 30 min; (B) Quantitative analysis of PPT RNA-DNA hybrid cleavage.