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. 2015 Mar 16;3(3):e12333. doi: 10.14814/phy2.12333

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© 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Expression of proteins upstream of mTORC1 was not differentially altered by alcohol in response to muscle overload. The relative amount of phosphorylated and total Akt (A, B), PRAS40 (C, D), ERK (E, F), and REDD1 (G) were measured in the plantaris muscle after 14 days of muscle overload. Representative images are shown corresponding to the sample order presented graphically. *P < 0.05, indicates significant difference from Sham condition within that treatment. Values are expressed as means ± SE.