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. 2015 Mar 16;3(3):e12333. doi: 10.14814/phy2.12333

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© 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Markers of autophagy were differentially regulated by alcohol in response to muscle overload. The relative amount of phosphorylated and total ULK1 (A, B), total p62 (C), and LC3-II (D) were determined in the plantaris muscle 14-days after muscle overload. Representative images of each marker are provided along with a representative image for GAPDH which was used for normalization of p62 and LC3A/B-II expression. *P < 0.05, indicates difference from Sham condition within that treatment; ^P < 0.05, indicates difference from Control-Sham. Values are expressed as means ± SE.