Figure 5.

Src is involved in the progression of cells toward mitosis following reversal from the early block by DFO. SKNSH cells were plated in 24-well plates with CM with DFO. Twenty hours later the cells were released from the DFO block by adding 1 mL of RM and FACS was conducted at 18 h after reversal detailed below. (A) AZD 0530 was added 30 min prior to reversal (B) AZD was added 15 min after reversal (C) AZD added 30 min after reversal (D) AZD added 60 min after reversal (E) DFO was readded 15 min after reversal (with no added AZD) (F) DFO was readded 2 h after reversal (G) DFO was readded 4 h after reversal.